Ka hoʻoholo ʻana o ka ʻike o ka waikawa Oxalic i nā huaʻai a me nā mea kanu e ka High-Performance Liquid Chromatography (HPLC)
1. Kumumanaʻo Hoʻokolohua a me ke ʻAno Hana
Ma ke ʻano he waikawa organik maʻamau i loko o nā huaʻai a me nā mea kanu, hoʻopilikia pololei ka nui o ka waikawa oxalic i ka ʻono a me ka waiwai o ka meaʻai. Hoʻohana kēia hoʻokolohua i ka chromatography wai hana kiʻekiʻe i hoʻohuli ʻia (RP-HPLC). Ma lalo o nā kūlana pae neʻe waikawa, hoʻokaʻawale ʻia ka waikawa oxalic mai nā mea hoʻopilikia me ka hoʻohana ʻana i kahi kolamu chromatographic C18. Hoʻohana ʻia kahi mea ʻike ultraviolet i hoʻonohonoho ʻia ma 210 nm no ka nānā ʻana i ka quantitative, ma muli o nā ʻano omo UV o nā hui carboxyl i loko o nā molekala waikawa oxalic.
2. Ka Hoʻomākaukau ʻana i ka Piʻo Maʻamau a me ka Laʻana
Hoʻohana ʻia ke ʻano hoʻoheheʻe gradient e hoʻomākaukau i nā hopena maʻamau: e kaupaona pololei i 25.0 mg o ka oxalic acid dihydrate a hoʻoheheʻe i 25 mL me ka wai ultrapure e loaʻa ai kahi hopena waihona 1 mg/mL. Hoʻoheheʻe ʻia kēia i loko o kahi moʻo o nā hopena maʻamau ma 50, 100, 200, 400, a me 800 µg/mL, me kēlā me kēia ʻano i hoʻokomo ʻia i ʻekolu mau manawa.
No ka hoʻomākaukau ʻana i ka hāpana, hoʻohana ʻia ka unuhi ʻana me ke kōkua o ka microwave: e kaupaona i 5.00 g o ka homogenate huaʻai/mea kanu, e hoʻohui i 10 mL o ka hopena hydrochloric acid 0.1 mol/L, e mālama ma 60°C me ka microwave no 10 mau minuke, e kānana ma o kahi kānana membrane 0.45 µm, a e hōʻiliʻili i ka filtrate no ka hoʻāʻo ʻana.
3. Hoʻonui ʻia o nā Kūlana Chromatographic
ʻO ka pae neʻe he ʻōnaehana 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), me ka nui o ke kahe ʻana o 0.8 mL/min a me ka mahana kolamu o 35°C. ʻO ka hoʻoponopono ʻana i ka hapa acetonitrile e hōʻike ana i ka wā e ʻoi aku ai ka pae organik ma mua o 10%, e pōkole ka manawa paʻa o ka waikawa oxalic i lalo o 3 mau minuke, akā loaʻa ka piko o ka tailing. Ma lalo o nā kūlana i hoʻomaikaʻi ʻia, ʻo ka manawa paʻa o ka waikawa oxalic he 4.2 mau minuke, e hoʻokō ana i ka hoʻokaʻawale piha ʻana mai ka waikawa citric a me ka waikawa malic e pili ana (hoʻonā > 1.5).
4. Hōʻoia ʻana o ke ʻano hana
Hōʻike ka hōʻoia ʻana o ka laulā linear i kahi pilina linear maikaʻi ma waena o ka ʻāpana piko a me ka noʻonoʻo ʻana ma ka pae o 10-1000 µg/mL (R² = 0.9993). ʻO ka palena o ka ʻike ʻana (LOD), i hoʻoholo ʻia e ke ʻano ratio signal-to-noise, ʻo 0.5 µg/mL. I nā hoʻāʻo hana hou ʻana, ʻo ka RSD no nā hoʻoholo replicate ʻeono o ka hāpana spinach like he 1.8%. ʻO nā hoʻokolohua hoʻōla spike ma ʻekolu pae (80%, 100%, a me 120%) e loaʻa ai ka awelika o nā hoʻōla o 98.2%, 102.4%, a me 97.8%, kēlā me kēia, e hoʻokō ana i nā koi no ka loiloi quantitative.
5. Ka Nānā ʻana i nā Laʻana Maoli
Ua hoʻāʻo ʻia ʻeono mau huaʻai a me nā mea kanu i loaʻa ma ke kālepa: spinach (356 ± 12 mg/100 g), celery (215 ± 9 mg/100 g), tomato (18 ± 2 mg/100 g), ʻāpala (6 ± 1 mg/100 g), maiʻa (ʻaʻole i ʻike ʻia), a me ka broccoli (89 ± 5 mg/100 g). ʻO ka hoʻohālikelike ʻana me ke ʻano hana maʻamau aupuni (GB 5009.277-2016) e hōʻike ana ʻo ka ʻokoʻa ma waena o nā ʻano hana ʻelua he emi ma mua o 5%, e hōʻoia ana i ka hilinaʻi o kēia ʻano hana. Ma ke ʻano kūikawā, hōʻike ke ʻano hana HPLC i ka ʻike kiʻekiʻe ma mua o nā ʻano hana titration kuʻuna i ka wā e kālailai ai i nā laʻana haʻahaʻa.
6. Nā Memo Koʻikoʻi
E kāohi pono i ka waiwai pH i ka wā o ka mālama mua ʻana o ka laʻana. Inā ʻoi aku ka pH o ka extract ma mua o 3, e alakaʻi ka hoʻoheheʻe ʻana o ka calcium oxalate i nā hopena hoʻāʻo haʻahaʻa. Pono e hoʻomākaukau hou ʻia ka pae mobile i kēlā me kēia lā e pale aku i ka hoʻoheheʻe ʻana o ka paʻakai a me ka paʻa ʻana o ke kolamu. Ma hope o kēlā me kēia 20 inikini, e holoi i ke kolamu me ka acetonitrile-wai (30:70) no 30 mau minuke e pale aku i ka hōʻino ʻana o ka pono o ke kolamu. No nā laʻana i loaʻa ka pigment (e laʻa, kāpeti poni), ua ʻōlelo ʻia e hoʻohui i kahi ʻanuʻu hoʻomaʻemaʻe extraction solid-phase ma mua o ka inikini.
7. Nā Kuhikuhi Noi a me ka Hoʻonui ʻana
Hiki ke hoʻonui ʻia kēia ʻano hana i ka ʻike ʻana i ka waikawa oxalic i loko o nā laʻana olaola e like me ka mimi a me ke koko. Ma ka hoʻoponopono ʻana i ka haku mele ʻana o ka pae lawe (e laʻa, me ka hoʻohui ʻana i ka tetrabutylammonium hydroxide), hiki ke hoʻoholo ʻia ka waikawa oxalic a me kāna mau metabolites i ka manawa like. I ka hui pū ʻia me kahi mea ʻike spectrometry mass, hiki ke hoʻokumu ʻia kahi ʻano ʻike ʻoi aku ka pololei. I ka ʻoihana hana meaʻai, hiki i kēia ʻano hana ke hāʻawi i ke kākoʻo ʻikepili koʻikoʻi no ke koho ʻana i nā ʻano waikawa haʻahaʻa-oxalic a me ka hoʻonui ʻana i nā kaʻina hana kuke.
ʻO Zibo Anhao Chemical Co., Ltd. - ʻAkika Oxalic
ʻO Zibo Anhao Chemical Co., Ltd. kahi mea hoʻolako hilinaʻi a me ka mea hoʻopuka aku i ka waikawa oxalic kiʻekiʻe (pae ʻoihana, 99.6% min) mai Shandong, Kina. ʻO kā mākou waikawa oxalic he mea paʻa kristal keʻokeʻo, i ʻike ʻia hoʻi ʻo ka waikawa ethanedioic, me ke ʻano molekala C₂H₂O₄. Loaʻa iā ia ka maʻemaʻe kiʻekiʻe, nā haumia haʻahaʻa, a me ke ʻano paʻa ma nā ʻāpana, e hoʻokō ana i nā koi o ka lole, ka ʻili, ka metallurgy, ka hana ʻana i ka honua laha ʻole, ka hoʻomaʻemaʻe kemika, a me nā ʻoihana kūkulu hale.
Hāʻawi mākou i ka waikawa oxalic anhydrous a me ka dihydrate, i loaʻa i loko o nā ʻeke 25kg, nā ʻeke 50kg, a me nā ʻeke nui 1000kg. Me nā kaʻina hana QC koʻikoʻi, nā hōʻike hoʻāʻo piha, a me nā hoʻolālā hoʻolako maʻalahi, hōʻoia mākou i ka lawe paʻa a me ka hana mau no nā mea kūʻai honua. Ua hoʻokuʻu nui ʻia aku kā mākou huahana i Asia Hikina Hema, ʻEulopa, ʻApelika, a me ʻAmelika, e loaʻa ana ka ʻike no ka maikaʻi hilinaʻi a me ke kumukūʻai hoʻokūkū.
E koho iā Zibo Anhao Chemical no ka hoʻolako ʻana i ka waikawa oxalic hilinaʻi a me ka lawelawe kemika hoʻokahi kū ʻoihana.
E koho iā Zibo Anhao Chemical no ka waikawa oxalic maʻemaʻe kiʻekiʻe e hāʻawi ana i ka hana paʻa a me ka waiwai no kāu ʻoihana. E kāhea mai iā mākou no nā kauoha nui a i ʻole nā kikoʻī loea: info@anhaochemical.com
